Dr. SHI Xiangyan (史祥燕)

Associate Professor

Ph.D. in Biochemistry (Arizona State University in 2014)

Address: 1 International University Park Road, Longgang District,  Shenzhen, Guangdong Province, 518172，P.R. China.

Office: 379 main building

Email: xyshi@smbu.edu.cn

Google scholar:

https://scholar.google.com/citations?hl=en&user=AvdHnWMAAAAJ&view_op=list_works&sortby=pubdate

Recruiting: postdocs, PhD students, master students.  

RESEARCH INTEREST

1. Biochemistry, structural biology and biophysics of chromatin regulation and epigenetics.    

2. Biochemistry, structural biology and biophysics studies of HBV viral minichromatin regulation, viral entry and replication.

3. Developing novel techniques, primarily biological solid-state NMR and solution-state NMR, and its integration with other advanced techniques such as Cryo-EM and structural bio-informatics, for studying molecular properties of biosystems.  

FUNDINGS

1. 廣東省人才項目專項（2022年入選）

2. 深圳市人才項目專項（2021年入選）

3. 國家自然科學基金青年項目（項目負責人，2023-2025）

4. 廣東省普通高校重點領域?qū)ｍ棧椖控撠熑耍?022-2025）

5. 廣東省國際及港澳臺高端人才交流專項（項目負責人，2022-2024）

6. 廣東省普通高校特色創(chuàng)新團隊（團隊結(jié)構生物方向負責人，2022-2025）

SELECTED PUBLICATIONS

(*Corresponding author)

Q. Chen, L. Zhao, A. Soman, A.Y. Arkhipova, J. Li, Hao Li, Y. Chen, X. Shi*, Lars Nordenski?ld*, Chromatin liquid–liquid phase separation (LLPS) is regulated by ionic conditions and fiber length, Cells 11(2022), 3145.

X. Shi*, Z. Zhai, Y. Chen, J. Li, L. Nordenski?ld*, Recent advances in investigating functional dynamics of chromatin, Frontiers in Genetics 13(2022), 870640.

Y. Lu, B. Sharma, W.L. Soon, X. Shi, T. Zhao, Y.T. Lim, R.M. Sobota, S. Hoon, G. Pilloni, A. Usadi, K. Pervushin, A. Miserez*, Complete sequences of the velvet worm slime proteins reveal that slime formation is enabled by disulfide bonds and intrinsically disordered regions, Advanced Science (2022), 2201444.

B. Gabryelczyk*, R. Alag, M. Philips, K. Low, A. Venkatraman, B. Kannaian, X. Shi, M. Linder, K. Pervushin*, A.Miserez*, In vivo Liquid-liquid phase separation protects amyloidogenic and aggregation-prone peptides during overexpression in E. coli, Protein Science 31(2022), e4292

T. Seviour*, F.R. Winnerdy, W.L. Li, X. Shi, S. Mugunthan, G.S. Kohli, H.M. Shewan, J.R. Stokes, S.A. Rice, A.T. Phan, S. Kjelleberg*, The biofilm matrix scaffold of Pseudomonas aeruginosa contains G-quadruplex extracellular DNA structures, npj Biofilms and Microbiomes 7(2021), 1-12.

M. Shoaib, Q. Chen, X. Shi, N. Nair, C. Prasanna, R. Yang, D. Walter, K.S. Frederiksen, H. Einarsson, J.P. Svensson, C.F. Liu, K. Ekwall, M. Lerdrup*, L. Nordenski?ld*, C.S. S?rensen*, Histone H4 lysine 20 mono-methylation directly facilitates chromatin openness and promotes transcription of housekeeping genes, Nature Communications 12(2021), 1-16.

W. Zhen, J. Sun, X. Ning, X. Shi, C. Xue*, Polymeric carbon nitride with internal np homojunctions for efficient photocatalytic CO2 reduction coupled with cyclohexene oxidation. Applied Catalysis B: Environmental 298(2021), 120568.

Y. Lekina, S.S.H. Dintakurti, B. Febriansyah, D. B., J. Yan, X. Shi, J. England, T. White, J.V. Hanna*, Z. Shen*, The effect of organic cation dynamics on the optical properties in (PEA) 2 (MA)[Pb 2 I 7] perovskite dimorphs, Journal of Materials Chemistry C 9(2021), 17050-17060.

X. Shi*, C. Prasanna, A. Soman, K. Pervushin, L. Nordenski?ld*, Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA. Communications Biology 3(2020), 639.

C. Qian, W. Zhou, J. Qiao, D. Wang, X. Li, W.L. Teo, X. Shi, H. Wu, J. Di, H. Wang, G. Liu, L. Gu, J. Liu, L. Feng, Y. Liu, S.Y. Quek, K.P. Loh, Y. Zhao*, Linkage engineering by harnessing supramolecular interactions to fabricate 2D hydrazone-linked covalent organic framework platforms toward advanced catalysis. Journal of the American Chemical Society, 142(2020), 18138-18149.

H. Xu, Y. Luo, X. Li, P.Z. See, Z. Chen, T. Ma, L. Liang, K. Leng, I. Abdelwahab, L. Wang, R. Li, X. Shi, Y. Zhou, X.F. Lu, X. Zhao, C. Liu, J. Sun*, K.P. Loh*, Single crystal of a one-dimensional covalent organic framework. Nature Communications 11(2020), 1434.

X. Shi *, C. Prasanna, K. Pervushin, L. Nordenski?ld*, Solid-state NMR 13C, 15N assignments of human histone H3 in the nucleosome core particle. Biomolecular NMR Assignments 14(2020), 99-104 .

W. Zhen, X. Yuan, X. Shi, C. Xue*, Grafting molecular cobaltoxo cubane catalyst on polymeric carbon nitride for efficient photocatalytic water oxidation, Chemistry- An Asian Journal 15(2020), 1–8 ).

B. Gartosz, H. Cai, X. Shi (co-second author), S. Yue, P. J.M. Swinkels, S. Salentining, K. Pervushin*, A. Miserez*, Hydrogen bond guidance and aromatic stacking drive liquid-liquid phase separation of intrinsically disordered histidine-rich peptides. Nature Communications 10(2019), 5465 (Editors’ Highlights).

S. H. Hiew, H. Mohanram,  L. Ning,  J. Guo,  A. Sánchez-Ferrer,  X. Shi, K.Pervushin,  Y. Mu,  R, Mezzenga,  A. Miserez, A short peptide hydrogel with high stiffness induced by 310-helices to β-sheet transition in water, Advanced Science, 1901173(2019).

G. Polido, X. Shi ( co-first author), D. Xu, C. Guo, R. Thai, J.P. Patterson, N.C. Gianneschi, T.M.  Suchyna, F. Sachs, G.P. Holland*, Investigating the interaction of Grammostola rosea venom peptides and model lipid bilayers with solid-state NMR and electron microscopy techniques, BBA-Biomembranes, 1861(2019), 151–160. (BBA Special Collection).

X. Shi, C. Prasanna, T. Nagashima, T. Yamazaki, K. Pervushin, L. Nordenski?ld*, Structure and dynamics in the nucleosome revealed by solid–state NMR, Angewandte Chemie, 57(2018), 9734–9738.

X. Li, Q. Gao, J. Aneesh, HS. Xu, Z. Chen, W. Tang, C. Liu, X. Shi, KV Adarsh, Y. Lu*, K. P. Loh*, Molecular engineering of bandgaps in covalent organic frameworks, Chemistry of Materials, 30(2018), 5743–5749.

X. Shi, C.M. Rienstra*, Site-specific internal motions in GB1 protein microcrystals revealed by 3D 2H-13C-13C solid-state NMR spectroscopy, Journal of the American Chemical Society, 138 (2016), 4105–4119.

D. Xu, X. Shi, F. Thompson, W.S. Weber, Q. Mou, J.L. Yarger*, Protein secondary structure of Green Lynx spider dragline silk investigated by solid-state NMR and X-ray diffraction, International Journal of Biological Macromolecules, 81(2015), 171-179.

X. Shi, G.P. Holland, J.L. Yarger*, Molecular dynamics of supercontracted spider dragline silk fiber investigated by 2H-13C heteronuclear correlation NMR, Biomacromolecules, 16(2015), 852-859.

X. Shi, J.L. Yarger, G.P. Holland*, Elucidating proline dynamics in spider dragline silk using 2H-13C HETCOR MAS NMR, Chemical Communications, 50(2014), 4856-4859.

X. Shi, G.P. Holland, J.L. Yarger*, Amino acid analysis of spider dragline silk using 1H NMR, Analytical Biochemistry, 440(2013), 150-157.

X. Shi, J.L. Yarger, G.P. Holland*, Probing site-specific 13C/15N-isotope enrichment of spider silk with liquid-state NMR spectroscopy, Analytical and Bioanalytical Chemistry, 405(2013), 3997-4008.

X. Shi, J.L. Yarger, G.P. Holland*, 2H-13C HETCOR MAS NMR for indirect detection of 2H quadrupole patterns and spin-lattice relaxation rates, Journal of Magnetic Resonance, 226(2013), 1-12.

Q. Zhou, X. Shi, F. Xu, Q. Zhang*, M. He, W. Wang, Mechanism of OH-initiated atmospheric photooxidation of the organophosphorus insecticide (C2H5O)3PS, Atmospheric Environment., 43(2009), 4163-4170.

X. Qu, H. Wang, Q. Zhang*, X. Shi, F. Xu, W. Wang, Mechanistic and kinetic studies on the homogeneous gas-phase formation of PCDD/Fs from 2,4,5-tichlorophenol, Environmental Science & Technology, 43(2009), 4068-4075.

Q. Zhang*, X. Qu, H. Wang, F. Xu, X. Shi, W. Wang*, Mechanism and thermal rate constants for the complete series reactions of chlorophenols with H, Environmental Science & Technology, 43(2009), 4105-4112.

Q. Zhang*, S. Li, X. Qu, X. Shi, W. Wang*, A quantum mechanical study on the formation of PCDD/Fs from 2-chlorophenol as precursor, Environmental Science & Technology 42 (2008), 7301-7308.

Conference Abstracts

J. Yarger, X. Shi, C. Guo, A. Lazaric, B. Cherry, Using magnetic resonance techniques to probe the structure and dynamics in synthetic & natural spider silks, Abstracts of Papers of the American Chemical Society, 255(2018).

G. Polido, X. Shi, D. Xu, T. Doan, G.P. Holland, Probing the interaction of spider venom neurotoxins with the lipid bilayer using solid-state NMR, TEM and DSC, Abstracts of Papers of the American Chemical Society, 251(2016).

X. Shi, D. A. Berthold, C. M. Rienstra, Site-specific protein internal motions revealed by 2H solid-state NMR spectroscopy, Biophysical Journal, 110(2016), 154a.

L.A. Della Ripa, A. G. Coiffi, S. Phinney, X. Shi, T. V. Pogorelov, M. D. Burke, C. M. Rienstra, Atomic resolution studies of sterol interactions by solid-state NMR spectroscopy, Biophysical Journal, 106(2016), 36a.

X. Shi, C. Guo, G. P. Holland, NMR characterization of spider venom neurotoxin structure and interactions with lipid bilayers, Biophysical Journal, 106(2014), 294a.